Procedure

• Remove a leaf (3-4cm) or small pieces of leaf from the infected plant and add to the extraction bottle (1). Smaller pieces of leaf from several parts of the plant may give better results.
• Screw cap tightly and shake the extraction bottle vigorously for 20 seconds (2). Do not use too much leaf material or shake the extraction bottle for too long as this affects the test.
• Remove the lateral flow device (III) from the foil pack. If only using one device, reseal the pack with tape to protect the remaining device.
• Draw into the transfer pipette (4), about half of the liquid from the extraction bottle. 
• Add 2 drops to the sample well on the lateral flow device (5). Maintain the device in a horizontal position for the duration of the test. The drops should be absorbed in about 30 seconds and a blue dye should appear in the viewing window (IIIc). If this does not happen, add one more drop to the well. Do not add any more.
• Wait until the blue control line (IIIa) appears and read the test (2-3 minutes). If the control line does not appear, repeat the test with a new device